DYNLL1 targeting and local nuclear alignment in U2OS with a telomeric 12q regional pattern — reproducible data and analysis

Version 1.0.1 correction: the additional DYNLL1_U2OS_correction_v1.0.1.zip contains corrected Supplementary Figures 2, 3 and 5, their source data, an audit of all 12 HIF guide/replicate cells, and reproducible plotting code. Extract the unchanged v1.0.0 base archive, then overlay the correction archive in the same directory. The large base archive and its reproduction report are unchanged. ACAAGAA Rep2 has raw High=1480 and Low=0; it passes the across-replicate guide rule but has an unreliable replicate denominator. Four zero-denominator cells are now explicitly flagged. All 12 original ratios, original guide eligibility and primary spatial results are preserved. Reproducible data and analysis for a descriptive study of local nuclear alignment after DYNLL1 targeting in U2OS cells, alongside a telomeric 12q regional pattern. The primary analysis contains 7,875 native DNA fields, 875 wells, 67 selected genes and 335 gene/date contrasts across five acquisition dates. Dates reuse one reagent pool per gene and shared controls; independent targeting, rescue and the molecular mechanism remain unvalidated. The archive retains complete core focal/field inputs, all alignment definitions and sensitivity analyses, grouped conventional-profile predictions, the plate/rank audit, same-plate comparisons, all 88 figure-panel sources, 22 supplementary tables and an explicit 25-reference verification matrix. All selected genes, dates and nonconfirming results are retained. Editorial drafts and private review correspondence are excluded. Bulk raw JUMP images and the Cellpose-SAM checkpoint are linked by public URLs and hashes rather than bundled in full. Code mirror: https://github.com/subhajit-dutta-bio/dynll1-u2os-nuclear-alignment . Original analysis code is MIT licensed; original derived release outputs are CC BY 4.0. Upstream data and metadata retain their own terms, as described in THIRD_PARTY_NOTICES.txt. This is a data and code deposit, not a journal submission, wet-lab validation, or claim of acceptance.