Total contributions of donor-derived DNA in transconjugants.

The total number of base pairs in donor-derived segments was calculated for three transconjugant cohorts (itemized lists appear in Tables S2 and S4). The total DNA can be subdivided into DNA associated with the selected Kmr gene, esx1, and unselected DNA. Transconjugant cohorts are recipient-proficient F1 transconjugants (Figure 1); donor-proficient F1 transconjugants (Figure 2), for which esx1 was enriched by screening for donor function; and backcross transconjugants that are either donor or recipient-proficient (Figures 4 and 5). Donor percentages assume 7 Mb of DNA per transconjugant genome, whereas percentages for segments that spanned Kmr, esx1, or were transferred but not selected were calculated per the total amount of donor DNA transferred in that cohort.aThe percentage for esx1 segments in the backcrosses was calculated for the three donor-proficient derivatives.bUnselected segments are not contiguous with the donor-derived Kmr gene or esx1 locus.cOnly three of the transferred segments were unchanged from their ancestral F1 parental boundaries, with the remainder representing subdivided fragments of previously uninterrupted donor tracts.