Tomatidine Attenuates Inflammatory Responses to Exercise-Like Stimulation in Donor-derived Skeletal Muscle Myobundles

We assessed donor-specific response to tomatidine known to inhibit muscle atrophy and promote skeletal muscle hypertrophy. Bioinformatic analyses revealed that infusion of tomatidine during electrical stimulation modulated the IL-6/JAK/STAT3 pathway improving muscle synchronicity in young myobundles compared to those from older volunteers. CD56 myoblasts-derived from young active and old sedentary muscle biopsies, differentiated into functional myobundles, that exhibited classic skeletal muscle bio-functions, were treated with tomatidine. We report on the differences in gene expression profiling between the treated- vs non-treated young and old-derived myobundles . A minimum of three biological replicates was used for RNA seq.