Transcriptomic response dynamics of human primary and immortalized adrenocortical cells to ACTH and Angiotensin II. Transcriptomic response dynamics of human primary and immortalized adrenocortical cells to ACTH and Angiotensin II

RNA sequencing provides a transcriptome-wide view of what processes are activated and repressed during steroidogenesis. Here we performed an RNA-seq time series on primary human adrenocortical cells and H295R cells stimulated with ACTH or AngII (forskolin for H295R). We found that the ligand-induced changes in gene expression largely involved the same genes and similar timing. While stimulus-induced expression changes in H295R cells are recapitulated by primary cells, there are also expression changes unique to primary cells. Overall design: RNA sequencing of forskolin-treated H295R cells and AngII/ACTH-treated primary adrenocortical cells