RNA-seq of equine alveolar macrophages and blood monocyte-derived macrophages exposed to an inflammatory stimulus

The obejective was to investigate how equine alveolar macrophages (AMs) and monocyte-derived macrophages (MDMs) response to a stimuli which contained fungal spores, lipopolysaccharide, and silica microsphere (FLS), and whether different responses existed between AMs and MDMs. RNA-seq was used to profile gene expression of AMs and MDMs from six clinically healthy horses were incubated with and without FLS for six hours. Overall design: Differential gene expression analysis between control and FLS-exposed AMs, and MDMs, respectively. In additon, a comparison between AMs and MDMs in their gene expression change affected by FLS.