HLA typing of 96 samples in one run of the Illumina MiSeq

Unambiguous HLA typing is important in transplant matching and disease association studies. High-resolution HLA typing that is not restricted to the peptide-binding region can decrease HLA allele ambiguities. Cost and technology constraints have hampered high-throughput and efficient high resolution unambiguous HLA typing. We have developed a method for HLA genotyping that preserves the very high-resolution that can be obtained by next-generation sequencing (NGS) but also achieves substantially increased efficiency. HLA-A, B, C and DRB1 genotypes can now be determined for 96 individuals in a single run of the Illumina MiSeq.