Oobius primorskyensis strain:KRH-2016 | isolate:Oob-CN-2010 Raw sequence reads

A new species of egg parasitoid, Oobius primorskyensis Yao & Duan (Hymenoptera: Encyrtidae), was collected from the emerald ash borer Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) near Vladivostok, Russia. Morphological analysis indicate that this taxon is sufficiently distinct from Oobius agrili Zhang & Huang 2005 to merit species status. DNA sequence divergence between O. primorskyensis and O. agrili distinguishes these species. Based on mapping read sequences from one species to genes of the other species, 89 percent of the genes in O. primorskyensis had homologs in O. agrili, and 94 percent of the genes in O. agrili had homologs in O. primorskyensis. When reads from O. primorskyensis were mapped to genes of O. agrili, there were over 2 million sequence variants, including 169 long (=20 nucleotides) insertions/deletions and over 1 million single nucleotide polymorphisms. When reads from O. primorskyensis were mapped to genes of O. agrili, there were over 3 million sequence variants, including 221 long (=20 nucleotides) insertions/deletions and over 1 million single nucleotide polymorphisms. This sequence variation provides ample markers for distinguishing these two species. The new species is a natural enemy of A. planipennis in its native range and may be considered for introduction against A. planipennis in North America.