Chromatin interactions of the EVI1 oncogene in myeloid leukaemia (4C-Seq)

EVI1 expression is associated with poor prognosis in myeloid leukaemia. Aberrant expression can result from Chr.3q alterations, which cause juxtaposition of enhancers that induce EVI1 activation via long-range chromatin interactions. More often, however, EVI1 expression occurs unrelated to 3q alterations, and it is unclear if its expression underlies similar dependencies as 3q+ cells. As enhancers regulate promoters by physical interaction/chromatin looping, we explored if the EVI1 promoter in EVI1+3q- cells interacts with distally located chromatin and if these intereactions promote EVI1 expression. Overall design: For this sample set, we performed 4C-Seq using EVI1 promoter specific baits on leukaemic cells from CML patients and AML/CML cell lines. 4C-Seq was performed as previously described by Stadhouders, R. et al. (doi:10.1038/nprot.2013.018).