Identifying the genomic regions co-bound by ASCL1 and mSWI/SNF remodelers during neural differentiation [ChIP-seq]

We interfeered with the ASCL1-mSWI/SNF interaction: to abolish ASCL1 function, we knocked out ASCL1 in human iPSCs, while we used the BRM014 inhibitor to block the mSWI/SNF ATPase activity. We then performed ASCL1 ChIPseq in DIV24 WT and BRM014-treated neural cultures, and SMARCB1 ChIPseq in DIV24 WT and ASCL1 KO neural cultures, when ASCL1 expression is highest. We first identified the ASCL1 and SMARCB1 binding sites in DIV24 WT cultures, and then overlaped them to identify the ASCL1-SMARCB1 co-bound genomic regions. By comparing the ASCL1 binding profile in WT versus BRM014-treated cell, as well as the SMARCB1 binding profile in WT versus ASCL1 KO cells at the ASCL1-SMARCB1 co-bound regions, we were able to investigate the recruitment dynamics between ASCL1 and mSWI/SNF remodellers.