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Rat IUGR islet transcriptome sequencing at 2 time points

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NIAID Data Ecosystem2026-05-26 收录
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Intrauterine growth restriction (IUGR) increases the risk of developing type 2 diabetes during adulthood. At day 18 of gestation, we used bilatual uterine artery ligation (BUAL) to restrict nutrient supply to developing rat fetuses to produce IUGR pups. At 2 and 10 weeks of age pancreatic islets were isolated and total RNA extracted using Trizol. Samples with RNA integrity numbers greater than 7 were used to generate libraries Overall design: 2 experimental conditions (IUGR and Control) and 2 time points (2 and 10 weeks of age) 3 biological replicates

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2018-09-12
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