EphA4 and EphA7 are not upregulated in ventral limb mesenchyme upon mutation of En1.

(a) Expression of EphA4 is mainly found I dorsal limb mesenchyme in wildtype and En1-/- mutant embryos. Arrows point to small sites of ventral expression in the forelimb in both wildtype and mutant embryos. (b) EphA7 is expressed in axons targeting the dorsal and ventral limb (neurofilament), as well as in dorsal musculature and certain ventral structures at the plexus region and in the proximal limb (arrows) in wildtype and En1-/- mutant embryos. Scale bar equals 100μm for all panels (EPS)