Whole Transcriptome analysis for porcine alveolar macrophage infected by African Swine Fever Virus. Whole Transcriptome analysis for porcine alveolar macrophage infected by African Swine Fever Virus

African swine fever virus (ASFV) is one of the most devastating swine pathogens characterized by nearly 100% mortality in naive herds and was recently emerged the in China. In this study, we generated the expression profile of porcine alveolar macrophages (PAMs) infected with a high pathogenic ASFV (Pig/Heilongjiang/2018 (Pig/HLJ/18) ASFV). Our data indicated that ASFV infection lead to a strong inhibition of host immunity but promote chemokine-mediated signaling pathway and neutrophil chemotaxis. Moreover, ASFV infection can modulate the host miRNA involved regulation network, leading to a significant increase of host metabolism related genes and acceleration of virus replication. Furthermore, ASFV-derived viral small RNAs (vsRNAs) can target some host immune response related genes. In conclusion, our transcriptome-wide data provide some insights into the regulatory mechanism during ASFV infection. Overall design: MicroRNA and mRNA profiles of porcine alveolar macrophages (PAMs) at different time-points post ASFV infection. MicroRNA and mRNA datasets were generated by deep sequencing, in triplicate, using Illumina Nextseq CN500 and Hiseq Xten platforms, respectively.