<strong>Supporting data for thesis entitled " 3D Neuroblastoma Tumor Sphere Enhances Tumor Cells Migration and Drug Resistance"</strong>

The folder contains the supporting data for thesis entitled "3D Neuroblastoma Tumor Sphere Enhances Tumor Cells Migration and Drug Resistance". The subfolders are arranged according to each result chapters. The dataset includes summary files of live-cell imaging, RNA sequencing and cellular migration assay results. The study is divided into five parts: 1) evaluation of drug resistance between 2D monolayer cell culture vs 3D spheroids, 2) development of a bright-field stain-free 3D migration assay, 3) mechanistic study of molecular events underlying cellular migration in a MYCN-amplifed neuroblastoam cell line, 4) the transcriptomic changes associate with neuroblastoma 3D migration and 5) the pro-tumorigenic functions of the immune checkpoint molecule B7H3 in neuroblastoma. <br>本数据集文件夹包含题为《3D神经母细胞瘤肿瘤球促进肿瘤细胞迁移与耐药性》的论文的配套实验数据。子文件夹按各结果章节编排。本数据集涵盖活细胞成像（live-cell imaging）、RNA测序（RNA sequencing）以及细胞迁移实验（cellular migration assay）的结果汇总文件。本研究分为五个部分：1）2D单层细胞培养与3D肿瘤球的耐药性对比评估；2）明场无染色3D迁移实验体系的构建；3）MYCN扩增型神经母细胞瘤细胞系中细胞迁移相关分子事件的机制研究；4）与神经母细胞瘤3D迁移相关的转录组学变化；5）免疫检查点分子B7H3在神经母细胞瘤中的促肿瘤发生功能。

搜集汇总

背景与挑战

背景概述

该数据集为神经母细胞瘤研究论文的支持数据，包含活细胞成像、RNA测序和细胞迁移实验结果的总结文件，按论文结果章节组织。数据集聚焦于3D肿瘤球体模型对细胞迁移和耐药性的影响，研究涵盖五个关键部分，包括耐药性评估、迁移实验开发、分子机制探索和转录组分析，适用于癌症生物学和药物研究领域。