Gamma delta T cell response to Yersinia pseudotuberculosis
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https://www.ncbi.nlm.nih.gov/sra/SRP326337
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资源简介：
A multifunctional population of Vg4 T cells are inhibited by Yersinia pseudotuberculosis. Mesenteric lymph node (MLN) suspensions from L. monocytogenes infected mice were stimulated with 10 MOI of WT or YopJ^C172A Y. pseudotuberculosis for 24 hours. Antibiotics were given 2 hours post-stimulation and brefeldin A was added for the last 5-6 hours. Five hundred Vg1.1/2- CD44hi CD27- gd T cells from each stimulation were flow sorted and processed for RNA sequencing. In a separate experiment, MLN suspension from L. monocytogenes infected mice were stimulated with 1 MOI of WT Yptb-bla (Yop translocation reporter). Five hundred Yop+ or Yop- Vg1.1/2- CD44hi CD27- gd T cells were flow sorted and processed for RNA sequencing.
创建时间：
2021-11-17
