File S1 - Hyperthermophile Protein Behavior: Partially-Structured Conformations of <i>Pyrococcus furiosus</i> Rubredoxin Monomers Generated through Forced Cold-Denaturation and Refolding

Supporting information and figures. Figure S1, Production details, protein construct details, isoelectric point, molar extinction coefficient, amino acid sequence, and SDS-PAGE profile of purified PfRd. Figure S2, DNA sequencing electrophoretogram of PfRd, showing with arrows the boundaries marking the restriction sites Nde I and Xho I, used for cloning the PfRd-encoding gene into the pET23-a vector. Figure S3, TRI data. Figure S4, TRI data. Figure S5, TRI data. Figure S6, Time-trace of PfRd in 6 M Gdm.HCl at 100°C (top panel). Figure S7, Time-trace of PfRd in 6 M Gdm.HCl at 20°C (top panel). (DOCX)