<b>Mesenchymal stem cell-based apelin gene therapy improves pulmonary artery remodeling in monocrotaline-induced pulmonary hypertension through PI3K/AKT/eNOS and ERK1/2 signaling pathways</b>

In this study, lentivirus plasmid vectors were used to achieve overexpression of the APLN gene in WJ-MSCs. The recombinant plasmid vector pcSLenti-APLN was constructed and confirmed by gene sequencing. The information details of pcSLenti-EGFP-APLN and gene sequencing result of recombinant plasmid vector were provided in the datasheet.

本研究采用慢病毒质粒载体，在沃顿胶间充质干细胞（WJ-MSCs）中实现APLN基因的过表达。研究构建了重组质粒载体pcSLenti-APLN，并通过基因测序对其完成验证。重组质粒载体pcSLenti-EGFP-APLN的详细信息以及该重组质粒载体的基因测序结果，均已在数据表（datasheet）中提供。

搜集汇总

背景与挑战

背景概述

该数据集围绕间充质干细胞（WJ-MSCs）的基因疗法研究，通过构建并验证重组质粒载体pcSLenti-APLN，实现APLN基因的过表达，以探索其在改善肺动脉高压模型（由野百合碱诱导）中肺动脉重塑的作用。研究发现，这种疗法通过激活PI3K/AKT/eNOS和ERK1/2信号通路来发挥治疗效果，属于细胞生物学、呼吸疾病和心脏病学交叉领域。