Diamond annotation

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Annotation list
for Calanus finmarchicus reference transcriptome using
DIAMOND3.

Contigs were aligned with DIAMOND??3
on NR (2019-09-29), Swissprot and Trembl (2018-12) to retrieve
corresponding best annotations.

An annotation
matrix was then generated by selecting the best hit for each database
if: i) the percent of the query length covered by the alignment was
higher than 60% ; ii) the percent of the subject length covered by
the alignment was higher than 40%; iii) the percent of identity of
the alignment was higher than 40%.

File
diamond_annotation_206k.tsv is
the annotation list for Calanus
finmarchicus reference
transcriptome using DIAMOND3
(36,274 contigs with an annotation in at least one database
out of the 206,012).

File
diamond_annotation_76k.tsv is
the annotation list for the 76,550 contigs expressed with more than 1
CPM in the RNA sequencing Bioproject PRJNA628886 using
DIAMOND3 (22,527 contigs with an annotation in at least
one database out of the 76,550).

3DIAMOND:
version v0.9.22, parameters: -f 6 qseqid qlen qcovhsp pident score
evalue length sseqid slen stitle.

Ref: Buchfink,
B., Xie, C. & Huson, D. H. Fast and sensitive protein alignment
using DIAMOND. Nat. Methods 12, 59–60 (2015).

Related to bioproject: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA628886