DamID in intestinal tissue of young adults of C. elegans hermaphrodites to decipher the binding target sites for LET-418 and MEP-1

LET-418/Mi-2 is an ATP-dependent chromatin remodeler and is part of large complexes like the NuRD complex which are repressive complex and has a role in vulval development in Caenorhabditis elegans. Along with MEP-1, LET-418 also forms the MEC complex which functions in the post embryonic development of the nematode. Earlier studies have shown that there is extensive chromatin remodeling taking place in the intestinal tissue. This study is aimed at deciphering the binding targets of LET-418 and MEP-1 in the intestine using DamID technique. Around 4500 worms were collected from each genotype (let-418::dam, mep-1::dam and gfp::dam) in two replicates. Sequencing and data analysis was performed according to (GOMEZ-SALDIVAR et al. 2021)