The GdmCl chemical-denaturation parameters for the three PsTRXs at pH 7.0 and 25°Ca.
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aThe errors are fitting errors to the two-state model. The chemical-denaturations were only fully reversible (either monitored by CD or fluorescence) in PsTRXh1; the chemical-denaturation of PsTRXf was partially reversible (see text for details), and that of PsTRXh2 was irreversible.bThe change occurred at ∼2 M GdmCl, going from Ve = 11.95 ml at [GdmCl] = 1.8 M to Ve = 10.29 ml at [GdmCl] = 2.0 M (data not shown).
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2015-12-02
