MT_eukDB

Eukaryote-derived PolyA-extracted metatranscriptome (‘MT-eukDB’), originating from 423,409,432 raw Illumina reads (~63.5 Gbps) recovered from the fungal community that colonized milled switchgrass during rumen-incubation. After quality filtering and assembly of the raw reads, we identified a total of 4,581,844 expressed genes for which 4,550,231 (99.31%) were predicted to encode proteins. The assembled metatranscriptome was filtered against the genome of the available cultured fungi (per 2017) before the MT-eukDB. The purpose of MT-eukDB was to use the protein sequences as a database to identify peptides derived from uncultured rumen fungi within a generated metaproteome data.