Expression data from fractionated prostate epithelial cells (fetal and adult). Homo sapiens

Subset of adult prostate basal cells and fetal prostate epithelial cells have enhanced tubule-initiating capability in vivo. Features associated with this process may be co-opted in cancer cells We used microarrays to contrast gene expression profiles of fetal and adult tubule-initiating cells compared to basal and luminal epithelial cells that can be isolated from bening prostate tissue specimens. Overall design: FACS sorting of dissociated fetal and adult tissue specimens was performed with Epcam, CD44, and CD49f antibodies. The following populations were isolated for Gene expression analysis: Epcam+CD44- (fetal prostate epithelial cells/tubule-initiating cells), Epcam+CD44-CD49fHi (adult tubule-initiating cells), Epcam+CD44+CD49fHi (adult basal cells), Epcam+CD44-CD49fLo (adult luminal cells)