Identify miRNAs involved in ER stress in wheat using miRNA sequencing

We used dithiothreitol (DTT) and tauroursodeoxycholic acid (TUDCA) to induce or suppress ER stress in wheat cells, respectively, with the aim to reveal the molecular background of ER stress responses using miRNA sequencing. After sequencing, we revealed a total of 1,939 miRNAs involved in the process of ER stress response, including 1,740 known miRNAs and 199 novel miRNAs. Overall design: Experiments were established with three treatments: control (C), DTT (D), and DTT+TUDCA (T). Three independent biological replicates were performed, and a total of nine leaf samples were performed by miRNA sequencing. Small RNA libraries were constructed from 6 mixed RNA pools comprising RNA from three chemical treatment groups. For each treatment group, two mixed RNA pools were assembled from three independent biological replicates.