Thermodynamically infeasible cycles for the E. coli metabolic reaction network iAF1260.

The list provides the complete set of cycles turned out in the thermodynamic feasibility analysis of a sample of different randomly generated flux configurations. Plus (resp. minus) signs indicate that the reaction participates in the cycle in its forward (resp. backward) direction. (R) indicates that the corresponding reaction is putatively reversible according to [21]. Analyzing interdependencies in the above cycles, one sees that the directions of the putatively reversible fluxes SERt2rpp, GLUt2rpp, ACt2rpp, GLYCLTt2rpp, THRt2rpp, SUCOAS, PPAKr, PROt2rpp, CAt6pp and GLUABUTt7pp are in fact constrained. See Supporting Table S1 for abbreviations.