Deviations of RGC size percentiles determined by flatmount and flow cytometry.

Difference (%) = (volume diameter−area diameter)/area diameter×100%.aRGC area was obtained from the flatmount method by using an image-analysis software.bD(a, Q) was calculated from the circle area equation (eq. 1, 2× (area/3.1416)1/2).cApparent D(v, Q): the RGC diameter was estimated by substituting its FSC value into the calibration curve of the standard microspheres (y = 0.027 x+2.16, eq. 2, x = FSC value; y = diameter).dFSC value was obtained from the flow cytometric measurement of RGCs.eD(v, Q): the RGC diameter was estimated by substituting D(a, Q) into the linear equation.fCalculated FSC value: the FSC value was calculated from D(v) (eq. 2).