Differential Epithelial Responses to GII.3 HuNoV Infection in Jejunal Human Intestinal Organoids (HIOs)

We performed RNA-seq to compare the epithelial transcriptomic response to GII.3 HuNoV infection to gamma-irradiated inactivated HuNoV (gGII.3) and media controls in parental HIE lines (J2 and J4) and genetically modified HIOs (J2STAT1-KO and J4FUT2-KI), which support enhanced viral replication due to STAT1 knockout and FUT2 knock-in, respectively. HIEs were harvested and analyzed at 24 hours post-infection/treatment. NCBI grant: Grant ID: U19 AI144297 Consortium name: Texas Medical Center Genomic Center for Infectious Diseases (TMC GCID) URL: https://gcid.research.bcm.edu/ Overall design: HIOs from two different individuals and their genetically modified counterparts were used for total RNA sequencing