Effects of low-doses of antiandrogenic substances on Wistar Rat testes [miRNome analysis]

Three antiandrogenic substances (Flutamide, Prochloraz, Vinclozolin) were administered in aqueous preparations at 3 doses: i) Acceptable Daily Intake (ADI) ; ii) No Observed Adverse Effect Level (NOAEL) ; iii) Low Observed Adverse Effect Level (LOAEL) . Pregnant females (20/group) were treated by gavage from gestation day (GD) 6 until postnatal (PND) day 21. After weaning, the offspring received the same test-substance as their mothers by oral gavage. Twenty males were selected to be raised until the weaning (Subset 1, PND 21), the day of sexual maturation (Subset 2, puberty) or early adulthood (Subset 3, PND 83±2). At each subset, testes were used for transcriptome and miRNome analyses.