Comparative transcriptome analysis reveals metabolic differences between planktonic and biofilm of Actinobacillus pleuropneumoniae.

To reveal the transcriptional profiles of Actinobacillus pleuropneumoniae under biofilm and planktonic growth, we established a biofilm-forming culture method and constructed a mutant strain ?pga with defect in biofilm formation. Wild-type and ?pga mutant strains of Actinobacillus pleuropneumoniae strain 4074 were cultured in bottles with shaking for planktonic (WT_PK) and in microplates in static status for biofilm (WT_BF, ?pga), respectively. The bacteria in logarithmic growth period of different culture groups were collected for RNA seq. Overall design: The planktonic bacteria were obtained by culturing bacteria in bottles with shaking, and the biofilm were cultured in microplates in static status. Substances adhered to the bottom of the microplates were obtained as the biofilm layer or adhesion layer.