Cancer specific tissue-resident memory T cells expressed ZNF683 up-regulated by autocrine IFN-?in colorectal cancer

Tissue-resident memory T (Trm) cells express CD103 to be maintained in the local microenvironment and to be strongly involved in local immunity of epithelial tissue. Trm has been associated with cytotoxicity in pathologies of not only virus infection and autoimmune disease but also many cancers. Tumor infiltration by CD103+ Trm cells was associated with patient survival in colorectal cancer. Tumor infiltrating CD103+ Trm cells were comprised predominantly of CD8 T cells expressing cytotoxic activation and immune check-point molecules called exhausted markers. Therefore, we focused on ZNF683 as a marker of cancer-specific Trm. The expression of ZNF683 in Trm was up-regulated by TCR and IFN-? signaling that was positive feedback by autocrine IFN-?. These results indicate that IFN-? and TCR signaling and ZNF683 are involved in the activation of Trm in tumor and would be promising targets for regulation of cancer immunity. Overall design: Samples were obtained from colorectal cancer tissue and normal colon tissue in the intact area from two patients. CD45 positive cells in the sample were obtained by isolation and separation. These cells were targeted for single-cell RNA-seq analysis using the BD Rhapsody Single-Cell Analysis System