Julien Mouysset, Alexandra Deichsel, Sandra Moser, Carsten Hoege, Anthony A. Hyman, Anton Gartner, Thorsten Hoppe (2011) CIL:35146, Caenorhabditis elegans, early embryonic cell. CIL. Dataset

This time-lapse series illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of npl-4 through RNAi. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Time-lapse differential interference contrast (DIC) microscopy identified that downregulation of cdc-48, ufd-1, and npl-4 increases the cell division delay of P1 in comparison to AB, leading to a prolonged three-cell stage. The series corresponds to experiments shown in Fig. 1B and Movie 5 of Mouysset et al. Movie is CIL 25632 and original data file is CIL 35146.

本时序系列展示了经过RNA干扰处理，npl-4基因缺失的秀丽隐杆线虫（C. elegans）胚胎从早期分裂直至四细胞阶段的发育过程。P0受精卵的第一次胚胎分裂是不对称的，产生了前端的AB细胞和较小的后端P1细胞。这些细胞具有不同的发育命运和分裂时间，AB细胞分裂时间大约在P1细胞之前2分钟。时间序列差分干涉对比（DIC）显微镜观察到cdc-48、ufd-1和npl-4的下调增加了P1细胞的细胞分裂延迟，与AB细胞相比，导致三细胞阶段的延长。该系列与Mouysset等人在图1B和电影5中展示的实验相对应。电影编号为CIL 25632，原始数据文件编号为CIL 35146。