LRF genome-wide occupancy in CD4+ T cells
收藏NIAID Data Ecosystem2026-03-13 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE149993
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To examine whether LRF protein could bind cis-regulatory elements in the genes that LRF regulates. We performed Chromatin immunoprecipitation followed by deep-sequencing (ChIPseq) on activated T cells using an in vivo biotinylation and streptavidin pull-down approach. Splenic CD4+ T cells from Rosa26BirA animals were activated and transduced with pMRX-LRFbio-tag-IRES-Thy1.1 retrovirus, or with a control retrovirus expressing Thy1.1 only. Streptavidin immunoprecipitation from transduced cells were performed.
创建时间：
2022-06-29
