Exome sequencing of MLL-ENL transgenic Mus musculus

De-novo identification of secondary driver mutations in acute myeloid leukemia (AML) development was done in an inducible mouse model for MLL-ENL fusion gene. The activation of this oncogene has previously been shown to be sufficient to induce aggressive AML however there are indications of variations in disease on-set dependent which may reflect differences in the somatic mutational landscape. Here, pGMs with the fusion gene were sorted from donor mice and transplanted into multiple recipient mice thereafter leukemia was allowed to develop. Potential secondary mutations among somatic variants were filtered out for further validation.