Gene expression profile at single cell level of cultuted bovine mammosphere-derived epithelial cells (MDECs)

Bovine mammosphere-derived epithelial cells are a population with reported stem cell-like properties. We assessed these cells using scRNA-seq for markers of pluripotency and other features associated with the effects of their conditioned medium (CM). Overall design: Parenchymal tissue of a non-lactating cow (B. taurus) was isolated by means of a biopsy and was dissociated into single cells. MDECs were isolated using previously established protocols. Upon isolation of colonies and formation of a monolayer, the cells were analyzed using scRNA-seq. Protocol described in "Ledet, M.M., Vasquez, A.K., Rauner, G. et al. The secretome from bovine mammosphere-derived cells (MDC) promotes angiogenesis, epithelial cell migration, and contains factors associated with defense and immunity. Sci Rep 8, 5378 (2018). https://doi.org/10.1038/s41598-018-23770-z"