Mutation patterns predict drug response in acute myeloid leukemia

As part of a study correlating co-occurring mutations in Acute Myeloid Leukemia (AML) with drug sensitivity, we performed single cell mutation analysis to document the subclonal composition for 8 patient samples (blood or marrow mononuclear cells) of the 99 patients studied. We utilized the Tapestri Single Cell DNA plus Protein Sequencing Kit (MissionBio) and the 45-gene myeloid panel (MissionBio) for targeted sequencing. Single cells were encapsulated, lysed, and barcoded. DNA and protein libraries were prepared. DNA analysis and sequencing was performed by the University of California Irvine High Throughput DNA Sequencing Core (NCI Shared Resource) on an Agilent Bioanalyzer and Illumina NovaSeq 6000. A total of 294 million to 893 million reads were obtained for each sample. The... (for more see dbGaP study page.)