Gene expression profiles of primary human gall bladder epithelial cells with and without infection with S.paratyphi WT and ∆cdtB mutant for 2 and 7 days

We have successfully established organoid culture model for human and murine gall bladder (GB) epithelial cells allowing long-time culture of primary epithelial cells. This model was used to analyze factors influencing stemness and to study infection with Salmonella paratyphi and its toxin Cdtb. Primary cells were isolated from healthy tissue of surgery specimen taken from gall bladders of patients undergoing cholecystectomy for reasons not related to diseases of the gall bladder, and cultured as described in the accompanying publication. 2 Replicates of each condition (2D uninfected, 2D + WT S.paratyphi, 2D + deltaCdtB S.paratyphi, early and differentiated organoids) were generated. Total RNA was extracted from samples and hybridized on Agilent-8x60k Human custom arrays as dual-color hybridizations. Gall bladder epithelium cells were infected with S.paratyphi WT or ∆cdtB mutant for 2 or 7 days and hybridized on Agilent-4x44k Whole Genome Human arrays as single-color hybridizations.