Expression of PIK3CA WT and mutant colon cancer cells grown with (+ Gln)or without glutamine (-Gln)

We used human gene expression microarray to interrogate how glutamine deprivation differentially impact gene expession in isogenic PIK3CA mutant and WT cells. Two isogenic PIK3CA cell line pairs: DLD1 PIK3CA mut (with the WT allele knockout), DLD1 PIK3CA WT (with the mutant allele knockout), HCT116 PIK3CA mut (with the WT allele knockout), HCT116 PIK3CA WT (with the mutant allele knockoout), with or without glutamine (Gln) for 24 hours and total RNA were extracted for microarray analyses.