Next Generation Sequencing of Wild Type and PTPRO-/- ESCC tumoral Transcriptomes

To induce tumorigenesis in the esophagus, 100 mg/mL of the water-soluble carcinogen 4NQO (Sigma-Aldrich) was added to the drinking water of 6-week-old mice for 16 weeks. The mice were sacrificed when body weight loss >1/3; otherwise, they were sacrificed 12 weeks after completion of 4NQO treatment.Total RNA from murine esophageal squamous cell carcinoma (Ptpro-/- vs. Ptpro+/+, N=3/group) was extracted and purified, transcriptome profiles were generated by deep sequencing, using Illumina HiSeq 2500 sequencer. Transcriptome profiles of esophageal squamous cell carcinoma of 34-week old mice (Ptpro-/- vs. Ptpro+/+, N=3/group) were generated by deep sequencing, using Illumina HiSeq 2500 sequencer.