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Characterization of C3bot1E174Q-C2I.

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https://figshare.com/articles/dataset/_Characterization_of_C3bot1E174Q_C2I_/174553
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A. The recombinant fusion toxin C3bot1E174Q-C2I is depicted. B. Coomassie blue staining of GST-C3bot1E174Q-C2I (left lane) and C3bot1E174Q-C2I (right lane) after SDS-PAGE. GST-C3bot1E174Q-C2I was expressed in E. coli and GST was cleaved off with thrombin. C. Western blot analysis of C3bot1E174Q-C2I. C3bot1E174Q-C2I (100 ng) as well as C2I (100 ng) and C3 (100 ng) for control were subjected to SDS-PAGE, blotted and proteins were detected with specific antibodies against C2I and C3bot.
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2013-01-21
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