Primers and half-probes used for detecting either the positive or negative (replicative) strand of different honeybee viruses and virus species complexes through RT-MLPA.

The PCR sequence tags on each halfprobe are in lower-case letters, the non-specific stuffer sequences (for generating PCR products with pre-determined sizes) are shown in upper-case letters and the target-specific sequences are shown in underlined upper-case letters. Each RPO probe is 5′-phosphorylated (indicated by P-)to permit ligation of the 5′ end of the RPO to the 3′ end of the LPO.