Additional file 10: of Comparative analyses of glycerotoxin expression unveil a novel structural organization of the bloodworm venom system

Raw data and summary of qPCR experiments performed on three tissue types (putative venom glands [group A], pharyngeal lobes [group B], and posterior body wall [group C]) of G. tridactyla. sheet a Ct values determined per analyzed gene and technical replicate for 10 biological samples using the 7300 System SDS v.1.4 software (Applied Biosystems). sheet b and c The Fold Change (RQ, relative quantification) between biological groups (putative venom glands [A], pharyngeal lobes [B], and posterior body wall [C]) and p-values were calculated with the DataAssist™ v.3.01 software (Applied Biosystems). Two technical replicates per analyzed biological sample (n = 10) contribute to the calculations of one group. The single copy ribosomal genes rps3 and rps15a were used as normalizer genes. Software parameters: Maximum allowable Ct value, 40.0; Include max Ct values in calculations, Yes; Exclude outliers among replicates, Yes; Adjust p-values using Benjamini-Hochberg False Discovery Rate, Yes; Normalization method, Endogenous Control; Selected controls, rps15a and rps3. sheet b RQ-values and p-values calculated for the putative venom glands [A] and pharyngeal lobes [B] in comparison to the body tissue [C]. sheet c RQ-values and p-values calculated for the pharyngeal lobes [B] in comparison to the putative venom glands [A]. sheet d and e The Fold Change (RQ, relative quantification) between biological groups (putative venom glands [A], pharyngeal lobes [B], and posterior body wall [C]) per analyzed specimen (n = 10, biological samples). Two technical replicates were performed per analyzed specimen. The single copy ribosomal genes rps3 and rps15a were used as normalizer genes. sheet f Primer sets used for qPCR amplification of five GLTx specific gene fragments and two normalizer genes (rps3, rps15a). (XLSX 63 kb)