Nitrogen source of medium used in this study.
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Nitrate was deprived from the N6 medium and 3.5 mM (NH4)2SO4 was used as the nitrogen source in M1, M2, M3, M4 medium. 2 mM KNO2 or 20 mM glutamine was added in these medium if necessary. The other components composed of N6 basal medium (deprive of nitrogen source), 20 mM KCl, 800 mg/L casein hydrolysate, 600 mg/L proline, 2.0 mg/L 2,4-D, 3% sucrose, and 0.3% phytagel at pH 5.8. ‘―’ indicates the component was not added. The standard N6 medium was used as a control in this experiment.
创建时间：
2015-12-02
