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Fernan Federici, Jim Haseloff (2012) CIL:41733, Arabidopsis thaliana. CIL. Dataset

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Confocal micrograph showing the tissue structures within the leaf of an Arabidopsis thaliana seedling. The sample was fixed and stained with propidium iodide, which labels DNA, and imaged four years later. Over time, oxidation of the stain differentiates the fluorescent properties in different parts of the tissue so that they can be excited with distinct wavelengths of light from a confocal microscope. The researchers are using these techniques to investigate cellular architecture in plants and gene activity. The horizontal field width of this image is 200 microns. Wellcome Image Award 2012.

共聚焦显微镜图像展示了拟南芥幼苗叶片内的组织结构。样本经过固定和用碘化丙啶染色,该染料标记DNA,并于四年后进行成像。随着时间的推移,染料的氧化作用区分了组织不同部分的荧光特性,使得它们能够被来自共聚焦显微镜的特定波长的光激发。研究人员正利用这些技术来探究植物的细胞结构以及基因活性。该图像的水平视野宽度为200微米。荣获2012年威康图像奖。
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