Data underlying the publication: Gut bacteria-derived volatiles mediate the Drosophila melanogaster (Diptera: Drosophilidae) attraction

Bacteria-originated volatile molecules play a crucial role in chemical communications between insects, representing their promising application as odor bait traps in pest control. In this study, we investigated the behavioral preferences of the fruit fly Drosophila melanogaster (Diptera: Drosophilidae) towards the fermentation broth of seven gut-associated bacteria using trap choice assays. All seven bacterial fermentations significantly attracted adults and larvae compared to the medium control. We assessed the effects of bacterial fermentations on bayberry fruits’ olfaction attractiveness to fly adults and found that the bayberry fruits sprayed with fermentation broth from seven bacteria were all significantly more attractive to insects than the non-sprayed fruits, resulting in increased egg numbers. We also compared the attractive effect of bacterial fermentations with a sugar-vinegar mixture and a commercial odor-bait. The commercial odor bait proved more enticing than the unconcent..., , , # Data underlying the publication: Gut bacteria-derived volatiles mediate the *Drosophila melanogaster* (Diptera: Drosophilidae) attraction

[https://doi.org/10.5061/dryad.5tb2rbpc4](https://doi.org/10.5061/dryad.5tb2rbpc4)

## Description of the data and file structure

We examined the attractiveness of 5th-instar adults and 3rd-instar larvae by behavioural traps and double choice tests in a climate chamber using artificial recordings. Adult choices were identified based on the number of adults entering the trap-choice device after 12 hours. Larval choices were identified based on first substrate contact and the number of larvae present in a substrate over time. Oviposition behaviours were identified based on the number of eggs entering the trap-choice device after 36 hours.

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