Analysis of global gene expression using RNA-sequencing reveals new roles of Yanghe Pingchuan decoction in asthma

Context: Yanghe Pingchuan decoction (YPD) has been used for asthma treatment for many years in China. Objective: We sought to understand the role of YPD, and find more potential targets for YPD-based treatment of asthma. Materials and methods: An ovalbumin-induced asthma model in rats was created. Staining (hematoxylin and eosin, Masson) was used to evaluate the treatment effect of YPD. RNA-sequencing was carried out to analyze global gene expression, and differentially expressed genes (DEGs) were identified. Analysis of the functional enrichment of genes was done using the Gene Ontology database. Analysis of signaling-pathway enrichment of genes was done using the Kyoto Encyclopedia of Genes and Genomes database. Real-time reverse transcription-quantitative polymerase chain reaction was undertaken to measure expression of DEGs. Results: Pathology showed that YPD had an improvement effect on rats with asthma. RNA-sequencing showed that YPD led to upregulated and downregulated expression of many genes. The YPD-based control of asthma pathogenesis may be related to calcium ion (Ca2+) binding, inorganic cation transmembrane transporter activity, microtubule motor activity, and control of canonical signaling (e.g., peroxisome proliferator-activated receptor, calcium, cyclic adenosine monophosphate). Enrichment analyses suggested that asthma pathogenesis may be related to Ca2+ binding and contraction of vascular smooth muscle. A validation experiment showed that YPD could reduce the Ca2+ concentration by inhibiting the Ang-?/PLA/CaM signaling axis. Discussion and conclusion: Control of asthma pathogenesis by YPD may be related to inhibition of the Ang-?/PLA/CaM signaling axis, reduction of the Ca2+ concentration, and relaxation of airway smooth muscle. Overall design: Expression of differential genes after high-throughput sequencing in normal and model asthmatic rats