GhMYB44-mediated network regulating secondary cell wall (SCW) biosynthesis in cotton fiber

This project investigates the transcriptional network regulated by GhMYB44 and its downstream factor GhFSN43 during secondary cell wall (SCW) biosynthesis in cotton fiber. RNA-seq was performed on 20 days post-anthesis (DPA) fiber samples from GhMYB44 overexpression (OE), RNA interference (RNAi), and GhFSN43 overexpression (OE) and knockout (KO) lines, using Gossypium hirsutum cv. CRI24 as the wild-type control. In addition, DAP-seq reads of GhMYB44 were generated to identify its genome-wide DNA binding sites. These datasets together provide a comprehensive resource for understanding the molecular mechanisms underlying transcriptional regulation of SCW biosynthesis in cotton fibers.