Effect of A1 on the transcriptome of mesenchymal stem cells. Effect of A1 on the transcriptome of mesenchymal stem cells

To investigate whether pharmacological stimulation of mesenchymal stromal cells (MSCs) could induce cross-presentation abilities, we treated MSCs with A1. We performed gene expression profiling analysis using data obtained from RNA-seq of untreated MSCs, MSCs treated only with the molecule A1, and MSCs treated with the molecule A1 and the model antigen ovalbumin. A1 is an Accum™ variant, a molecule design to enhance intracellular accumulation of associated molecules through endosomal disruption. Endosomal break was targeted for its crucial role in antigen export to the cytosol and cross-presentation of endocytosed antigen. Overall design: Comparative gene expression profiling analysis of RNA-seq data for untreated mesenchymal stromal cells and A1-treated cells, co-treated with the antigen ovalbumin, or not.