Expression data of peritoneal macrophages from Wild-type mouse cultured in control or serine/glycine-depleted medium.

Activation of signaling pathways downstream of Toll-like receptor 4 upregulate expression of genes related to serine metabolism. We cultured peritoneal macrophages in control or serine/glycine-depleted medium for 24 hr, stimulated them with LPS (10 ng/ml) for 6 or 12 hr, and collected them to compare the gene expression. Microarray analysis revealed that serine/glycine-depletion in peritoneal macrophages upregulates gene expression of enzymes for serine biosynthesis. We obtained primary peritoneal macrophages from 3% thioglycollate-treated mice 4 days after the injection, cultured them in control or serine/glycine-depleted medium for 24 hr, stimulated them with LPS (10 ng/ml) for 6 or 12 hr, and collected them for RNA extraction and hybridization on Affymetrix microarrays.