Vakrakou A., Paschalidis N., et al 2023 Deep immunophenotyping of PBMCs from MS patients

We designed a novel 44 -parameter mass cytometry panel to interrogate the role of effector and regulatory subpopulations of peripheral blood myeloid subsets along with B and T cells (excluding granulocytes) in MS, assessing three different patient cohorts: RRMS, PPMS (Primary Progressive) and Tumefactive MS patients (TMS)
Conclusion:
We have identified previously uncharacterized subsets of circulating myeloid cells and shown them to correlate with distinct disease forms of MS as well as with specific disease state (relapse / remission). These results highlight the potential for therapies that specifically target myeloid cells to impact MS activity and progression depending on the disease subtype and stage of the disease. An optimized multiplex flow cytometry protocol for the analysis of intracellular signaling in peripheral blood mononuclear cells