Transcriptome Analysis of ER Stress-Related Genes in Cyclocarya paliurus

Cyclocarya (Juglandaceae) is a genus comprising only one species (C. paliurus (Batal.) Iljin.), which is considered an endangered tree species in China . It belongs to three types of protected plants and is mainly distributed in Hunan, Jiangxi, Anhui, and Guizhou.we investigated the genetic mechanisms of ER stress in C. paliurus by performing transcriptome analysis to compare the profiles of ER stress-related gene expression via different time-course Tunicamycin (TM) treatment(0 hour, 6 hour,14 hour). A total 196,207 unigenes were detected, with 1,867 down-regulated and 3,040 up-regulated differentially expressed genes (DEGs) found in both treatment (TM_6h and TM_14h )compare to control(TM_0h). Overall design: The 0.2 gram leaves from three individual trees were treated with tunicamycin (TM, 5 µg/mL) and Triton (0.5%, to improve leaf uptake) for 0 hours, 6 hours, and 14 hours(TM_0h, TM_6h, and TM_14h. Novogene (Beijing, China) in triplicate,performed Illumina Hiseq apparatus sequencing for this study,