Transcriptome analysis of CMT64 cells following TGF-ß and/or TNF-a treatment.

We have previously demonstrated that TNF-a, a proinflammatory cytokine, enhances TGF-ß-mediated EMT in A549 human lung cancer cells. RNA-sequencing analysis on CMT64 cells following TGF-ß and/or TNF-a treatment revealed a subset of genes possibly regulated by TGF-ß and/or TNF-a. Overall design: CMT64 cells were stimulated with 5 ng/ml of TGF-ß and/or 10 ng/ml of TNF-a, and RNA-sequencing was performed ugsing Illumina HiSeq.