<b>Metadata for the article "</b><b>Interplay of biotic and abiotic factors shapes tree seedling growth and root-associated microbial communities"</b>
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Root-associated microbial communities can alleviate plant abiotic stresses, thus potentially supporting adaptation to a changing climate during range expansion. While climate change is extending plant species fundamental niches northward, the distribution and colonization of mutualists (e.g., arbuscular mycorrhizal fungi) and pathogens may constrain plant growth and regeneration. Yet, the degree to which biotic and abiotic factors impact plant performance and associated microbial communities at the edge of their distribution remains unclear. In this study, we used a combination of root microscopy and amplicon sequencing to characterize tree root microbial communities (soil and root mycorrhizae, bacteria, and fungi) of sugar maple seedlings. We also explored the affect of abiotic and biotic factors on root microbial communities and their relationship with seedling growth along two elevation gradients in Quebec, Canada.<br>Metadata:comm_16S_2023: Bacteria ASV table with sequence counts per sample comm_ITS_2023: Fungi ASV table with sequence counts per sample comm_AMF_2023: Arbuscular mycorrhiza fungi (AMF) ASV table with sequence counts per sample taxa_16s_2023: Bacteria taxonomy taxa_ITS_2023: Fungi taxonomytaxa_AMF_2023: AMF taxonomy fungi_guild_meg: Table containing functional assignments for fungi described from one of the elevation gradientsfungi_guild_sut: Table containing functional assignments for fungi described from one of the elevation gradientsMetadata_clean_2023: Table containing information on the variables used in the modelling. Contains numerical data behind Figure 2 in the article, such as seedling annual growth (ann_growth) and elevation (alt).
与植物根系关联的微生物群落可缓解植物非生物胁迫,进而在物种分布区扩张过程中潜在辅助植物适应气候变化。尽管气候变化正将植物物种的基础生态位向北推移,但互惠共生生物(例如丛枝菌根真菌 (arbuscular mycorrhizal fungi))与病原菌的分布及定殖,可能会限制植物的生长与自然更新。然而,在物种分布边缘地带,生物与非生物因素对植物表现及其相关微生物群落的影响程度仍有待阐明。 本研究结合根系显微镜观测与扩增子测序技术,对加拿大魁北克地区两条海拔梯度上的糖枫幼苗根系微生物群落(涵盖土壤与根系菌根、细菌及真菌)进行了表征。同时,本研究还探究了非生物与生物因素对根系微生物群落的影响,以及这些因素与幼苗生长之间的关联。 元数据 (Metadata): comm_16S_2023: 细菌扩增子序列变异 (Amplicon Sequence Variant, ASV) 表,包含各样本的序列计数 comm_ITS_2023: 真菌扩增子序列变异 (Amplicon Sequence Variant, ASV) 表,包含各样本的序列计数 comm_AMF_2023: 丛枝菌根真菌 (arbuscular mycorrhizal fungi, AMF) 扩增子序列变异 (Amplicon Sequence Variant, ASV) 表,包含各样本的序列计数 taxa_16s_2023: 细菌分类学注释表 taxa_ITS_2023: 真菌分类学注释表 taxa_AMF_2023: AMF分类学注释表 fungi_guild_meg: 包含其中一条海拔梯度所获真菌功能注释信息的表格 fungi_guild_sut: 包含另一条海拔梯度所获真菌功能注释信息的表格 Metadata_clean_2023: 包含建模所用变量信息的表格,涵盖论文图2背后的数值数据,例如幼苗年生长量(ann_growth)与海拔(alt)。




